Whole-Mount X-Gal Staining of Mouse Tissues
نویسندگان
چکیده
منابع مشابه
Clearing and photography of whole mount X-gal stained mouse embryos.
Advances in genetic manipulation in mice have made knockout, knock-in, and transgenic mice highly useful for elucidation of gene function and structure. In many cases the mice are engineered to include a reporter gene, for example, β-galactosidase (lacZ) or green fluorescent protein (GFP). The reporter gene enables visualization of the expressing cells and tissues in both the heterozygous and m...
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Knowing expression patterns of given proteins is very important to understand their functions. Immunostaining analysis with specific antibodies is commonly used to identify cells or tissues expressing proteins of interest. Although this technique is regularly used, it requires high quality of specific antibodies and there is no good quality of antibody available for certain proteins. Alternativ...
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This protocol comprises the entire process of immunofluorescence staining on mouse cochlea whole mount, starting from tissue preparation to the mounting of the tissue. This technique provides "three-dimensional" views of the stained components in order to determine the localization of a protein of interest in the tissue in its natural state and environment.
متن کاملA novel protocol of whole mount electro-immunofluorescence staining
PURPOSE To develop a new method of whole mount immunostaining that improves the penetration of staining reagents into the cornea and decreases non-specific binding and background. METHODS Adult mouse corneas were fixed overnight in 4% paraformaldehyde or a mixture of 4% paraformaldehyde and 0.2% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4, at 4 degrees C. After washing with 0.1% Triton X...
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INTRODUCTIONThe technique presented this protocol is ideal for obtaining an overall, three-dimensional picture of the distribution of an antigen in embryos from the prestreak stage to ~10.5 dpc (days post-coitum). The embryos are fixed and permeabilized so that antibodies can penetrate all of the tissues. However, antibodies will not penetrate the embryo completely if it is too large, and so it...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2014
ISSN: 1940-3402,1559-6095
DOI: 10.1101/pdb.prot073452